Toxic Effects of Ethonol Treated Wistar Rats and Remedial Role of Green Tea (Camellia Sinensis)

Abstract

The current study attempted to evaluate Camellia sinensis's preventive benefits against
ethanol toxicity. A number of researchers have studied C. sinensis for its antioxidant,
anticarcinogenic, antiangiogenic, antiviral, and bactericidal effects. Green tea's polyphenolic
components, particularly epigallocatechin-3-gallate (EGCG), are thought to mediate many of
its benefits. Herbs and medicinal plants have been used for ages to cure numerous ailments
across the world, and 80 percent of the world's population1relies on botanical1preparations as
medication for their1health needs. A natural product's biological activity is frequently
thought to be the consequence of the combined action of multiple of its parts. Fruits,
vegetables, popular drinks, cereals, numerous marine items, medicinal plants, and herbs have
a wide range of pharmacological activities and include nutraceuticals that have the ability to
protect against a variety of illnesses. To achieve the current study's goal, two-month-old
Wistar rats (Wistar strain) were employed for the studies. Wistar rats of recognized breed
measuring 100-150g body weight were obtained from a certified animal producer. The rats
were provided a regular diet and had unlimited access to water. Their typical diet comprised
of carbs (69%), proteins (16%), lipids (7%), fiber (6%), and a vitamin-enriched mineral salt
combination (2%). The1green tea leaves were used to make an aqueous extract of Camellia
sinensis. Using a mortar and pestle, the tea leaves were pounded into powder. With the use of
a soxhelet apparatus with thermostatically controlled heating mental, an extract of 15
powdered leaves was made in distilled water. The experimental animals were weighed and
maintained in separate plastic cages. Every day between 10 a.m. and 11 a.m., each rat was
given ethanol orally. The body weights of all animals1were recorded at end of experiment.
1The animals were subsequently dissected & killed on time, under mild ether anesthesia.
Retro-orbital plexus was used to collect blood samples in fluoride and plain glass tubes. For
biochemical testing, the liver was taken, weighed, and processed. Blood samples1from rats
were immediately1centrifuged at 3000 rpm for 10 minutes at 4 0C to produce the
serum1samples.