The correlation between vitamin D receptor (FokI SNP (rs2228570) C to T and IL-6 levels with AMH in polycystic ovarian syndrome in Iraqi Women

Abstract

Objective: This study compared the variations in some clinical parameters between patients
with polycystic ovary syndrome and controls, as well as the genotyping for vitamin D receptors
FokI SNP (rs2228570) C to T.
Methods
Twenty-six plus 38 women diagnosed with PCOS were enrolled in the study. The
research period has been extended from December 2021 to May 2022. After 10-12 hours of
fasting, 5ml of blood was drawn from each patient and healthy human via vein puncture with
5ml disposable syringes. Two aliquots of blood were taken out. For DNA extraction, the initial
aliquot (2 ml) was put into an EDTA tube. Using the second aliquot (3ml) of the separated
serum, tests for AMH and IL-6 levels.
Results
Results showed a variety in genotyping frequency, with CC genotype ratio in controls
being as a reference and in PCOS patients. TT genotype ratio in PCOS patients was high
significant than in controls, and significant differences between study groups were found (p =
0.004), OR>1 (OR=10.3) denotes a genotype pattern that raises the risk factor for PCOS. While
(OR = 1.8) for the CT genotype in PCOS when compared to the control group; shows nonsignificant
between PCOS and controls, the P value was (p = 0.5). The results of allele
frequency for discovered T revealed that the differences between PCOS and normal control
were highly significant, with a p value of (p=0.0001). Where the odds ratio (OR=7.2) to allele
(T>1) is greater than one, this ratio represents allele T as a risk allele (risk factor).
Conclusion
Vitamin D is inversely related to AMH. This suggests that chronic low vitamin D levels
may have a more lasting negative effect on ovarian reserve. Should be investigated to see if it
contributes to the reduction of According to the ROC curve results, measuring both AMH and
IL-6 together was more useful in distinguishing between women with PCOS and women
without PCOS.